Part:BBa_K741003

pRM-RBS-GFP-T

Promoter pRM with GFP gene downstream. The pRM is a modified one, whose OR3 (the third right operator) is mutated to be obliterated. This means our pRM is relatively difficult to be repressed by Cro dimer and can always be activated by CI dimer. We use this part to test the basic level of expression of modified pRM.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 752
  

Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.

Data

pRM-RBS-GFP-T
OD
t/min	Parallel	Experimental group	M9 medium
0	1	0.0593	0.045
	2	0.0642
	3	0.0678
45	1	0.0605	0.0436
	2	0.0734
	3	0.062
90	1	0.0648	0.0435
	2	0.0735
	3	0.0702
135	1	0.0811	0.0447
	2	0.0716
	3	0.0677
180	1	0.0723	0.0446
	2	0.0828
	3	0.0789
pRM-RBS-GFP-T
fluorescence intensity
t/min	Parallel	Experimental group	M9 medium
0	1	19.55	11.801
	2	19.571
	3	19.352
45	1	21.548	13.386
	2	20.505
	3	19.107
90	1	24.441	11.541
	2	23.777
	3	24.503
135	1	28.938	11.894
	2	27.884
	3	27.81
180	1	41.376	11.066
	2	39.573
	3	39.795